Noninvasive Detection of Echinococcus multilocularis Tapeworm in Urban Area, Estonia
ثبت نشده
چکیده
Samples were collected and stored at −80°C for ≥1 week to avoid the risk of infection with any Echinococcus spp. eggs present. Approximately 250 mg was placed into 2-mL tubes, heated at 65°C for 15 min, and stored at −80°C. The heating and cooling procedure helps to break the parasite egg shells, enabling more efficient DNA extraction. DNA was extracted by using the QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
منابع مشابه
Noninvasive Detection of Echinococcus multilocularis Tapeworm in Urban Area, Estonia
To the Editor: Alveolar echinococcosis, which is caused by the fox tapeworm Echinococcus multilocularis, is an emerging disease in Europe that shows a high mortality rate (1). Humans can become infected after ingesting parasite eggs (e.g., through direct contact with dogs and red foxes [Vulpes vulpes] or with their contaminated feces). E. multilocularis tapeworm eggs are extremely resistant and...
متن کاملWilderness in the city: the urbanization of Echinococcus multilocularis.
A distinct increase in fox populations, particularly in urban areas, has been observed in Europe. This is of particular concern in endemic regions of the small fox tapeworm Echinococcus multilocularis, the aetiological agent of human alveolar echinococcosis. Novel tools have facilitated the investigation of the ecology of urban foxes and have demonstrated the urban wildlife cycle of E. multiloc...
متن کاملInvestigation on the occurrence of Echinococcus multilocularis in Central Italy
BACKGROUND Recent studies on geographic distribution of Echinococcus multilocularis in Europe show that it has a wider range than previously thought. It is unclear, however, if the wider distribution is due to its recent spreading or to a lack of previous data from the new areas. Italy, previously considered E. multilocularis-free, is now part of these new areas: infected foxes (the main defini...
متن کاملSevere Delayed Hemolysis Associated with Regulated Parenteral Antimalarial Drug
Techapp1.pdf) were designed to amplify short sequences of mitochondrial DNA. On the basis of primer specificity and amplicon size, we determined host and parasite species (online Technical Appendix Figure 1). DNA extraction and PCR were performed in a laboratory dedicated to environmental samples (for a complete description of methods, see online Technical Appendix). DNA was successfully extrac...
متن کاملStability of the southern European border of Echinococcus multilocularis in the Alps: evidence that Microtus arvalis is a limiting factor.
SUMMARY The known range of the zoonotic fox tapeworm Echinococcus multilocularis has expanded since the 1990s, and today this parasite is recorded in higher abundances throughout large parts of Europe. This phenomenon is mostly attributed to the increasing European fox populations and their invasion of urban habitats. However, these factors alone are insufficient to explain the heterogeneous di...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2014